1 00:00:15,316 --> 00:00:23,036 Speaker 1: Pushkin. If you took a COVID test recently, you probably 2 00:00:23,036 --> 00:00:26,076 Speaker 1: took it at home, and if you tested positive, you 3 00:00:26,156 --> 00:00:29,356 Speaker 1: probably didn't report the results to your local health department, 4 00:00:29,636 --> 00:00:32,756 Speaker 1: which you know fair enough, no judgment. But in the 5 00:00:32,756 --> 00:00:36,156 Speaker 1: big picture this does create a problem. How do we 6 00:00:36,276 --> 00:00:40,476 Speaker 1: keep track of COVID levels as public testing declines. How 7 00:00:40,476 --> 00:00:42,396 Speaker 1: do we look out for new variants? How do we 8 00:00:42,556 --> 00:00:47,276 Speaker 1: monitor new outbreaks? The answer, in a word, is poop. 9 00:00:52,316 --> 00:00:54,996 Speaker 1: I'm Jacob Goldstein and this is what's your problem. My 10 00:00:55,036 --> 00:00:58,956 Speaker 1: guest today is Mariana Matus, co founder and CEO of 11 00:00:59,076 --> 00:01:04,036 Speaker 1: biobot Analytics. The company analyzes sewage samples from hundreds of 12 00:01:04,076 --> 00:01:07,436 Speaker 1: water treatment plants across the US and Canada. They provide 13 00:01:07,436 --> 00:01:10,556 Speaker 1: an aggregate picture of the cod status of tens of 14 00:01:10,676 --> 00:01:14,956 Speaker 1: millions of people. Marianna's problem is this, how do you 15 00:01:15,036 --> 00:01:18,556 Speaker 1: move beyond COVID to turn raw sewage into data that 16 00:01:18,596 --> 00:01:24,076 Speaker 1: can help improve global public health. As it's become clear 17 00:01:24,076 --> 00:01:26,236 Speaker 1: that sampling sewage is one of the best ways to 18 00:01:26,276 --> 00:01:29,996 Speaker 1: track the COVID pandemic, the field of wastewater epidemiology has 19 00:01:30,076 --> 00:01:33,236 Speaker 1: become a big deal. But when Marianna discovered the field, 20 00:01:33,356 --> 00:01:36,956 Speaker 1: it wasn't particularly popular or flashy. It was years before 21 00:01:36,956 --> 00:01:39,796 Speaker 1: the pandemic when she was working on her PhD and 22 00:01:39,916 --> 00:01:45,596 Speaker 1: computational biology at MIT. But Marianna told me studying wastewater 23 00:01:45,716 --> 00:01:48,196 Speaker 1: seemed like a great fit for what she wanted to do. 24 00:01:48,596 --> 00:01:52,796 Speaker 1: When I came across this space of voicewatermology through through 25 00:01:52,796 --> 00:01:56,436 Speaker 1: my laboratory, it was perfect, I think for me as 26 00:01:56,556 --> 00:02:01,156 Speaker 1: a combination of cutting edge science but also like a 27 00:02:01,316 --> 00:02:06,716 Speaker 1: very big societal impact. When you imagined having a big 28 00:02:06,756 --> 00:02:11,436 Speaker 1: societal impact through your study of wastewater epidemiology, like what 29 00:02:11,556 --> 00:02:15,596 Speaker 1: did you imagine? What was like your big, big dream. 30 00:02:15,716 --> 00:02:18,796 Speaker 1: I grew up in Mexico City and in a you know, 31 00:02:18,836 --> 00:02:21,836 Speaker 1: in a very sort of like slummy part of Mexico City, 32 00:02:21,916 --> 00:02:25,076 Speaker 1: not on the nice part of Mexico City. So I 33 00:02:25,076 --> 00:02:33,556 Speaker 1: grew up basically with zero government resources, you know, in 34 00:02:33,636 --> 00:02:37,356 Speaker 1: areas that are ignored and that don't receive any sort 35 00:02:37,356 --> 00:02:41,476 Speaker 1: of like government resources. And I grew up thinking that 36 00:02:41,636 --> 00:02:45,196 Speaker 1: life was like that. And then my career took me 37 00:02:45,236 --> 00:02:49,196 Speaker 1: to study in great universities and places, you know, in 38 00:02:49,236 --> 00:02:52,676 Speaker 1: the Netherlands, in the UK, in Boston, and then I 39 00:02:52,716 --> 00:02:56,676 Speaker 1: just began to realize that it actually it's not like that. 40 00:02:56,796 --> 00:02:59,756 Speaker 1: Not every places like that, And to me, you know, 41 00:02:59,796 --> 00:03:01,836 Speaker 1: I think like the more that dug into it through 42 00:03:01,876 --> 00:03:05,796 Speaker 1: my peach D studies, like I realized that a big 43 00:03:05,836 --> 00:03:09,956 Speaker 1: part of the solution starts with collecting the data to 44 00:03:10,116 --> 00:03:14,356 Speaker 1: demonstrate those health disparities that you know, I know that 45 00:03:14,396 --> 00:03:16,316 Speaker 1: are out there and everybody sort of like knows that 46 00:03:16,356 --> 00:03:19,716 Speaker 1: are out there. But it's just hard to to really 47 00:03:19,796 --> 00:03:22,316 Speaker 1: take action when you just don't have it in your 48 00:03:22,316 --> 00:03:25,556 Speaker 1: face and not don't have a way to measure progress. 49 00:03:25,636 --> 00:03:28,516 Speaker 1: So what I would imagine is, how can we use 50 00:03:29,196 --> 00:03:32,516 Speaker 1: the data from the waste water to give everybody a 51 00:03:32,636 --> 00:03:37,916 Speaker 1: voice and to create maps of what's happening near real 52 00:03:37,996 --> 00:03:43,196 Speaker 1: time everywhere and to and to design action around that data. 53 00:03:43,316 --> 00:03:45,676 Speaker 1: How did you go from that initial kind of big 54 00:03:45,796 --> 00:03:49,276 Speaker 1: dream to starting a company? So when I came to MT, 55 00:03:49,756 --> 00:03:51,956 Speaker 1: it sort of like I had all these dreams in 56 00:03:51,996 --> 00:03:56,116 Speaker 1: my head about like, oh, imagine using science to you know, 57 00:03:56,436 --> 00:04:00,476 Speaker 1: just impact society. Wouldn't that be amazing? But I didn't 58 00:04:00,556 --> 00:04:03,316 Speaker 1: know what that meant in practice. And when I came 59 00:04:03,356 --> 00:04:09,476 Speaker 1: to MT, I discovered entrepreneurship. Entrepreneurship, h yes, I mean 60 00:04:09,556 --> 00:04:13,036 Speaker 1: just like the concept that anybody can be a founder 61 00:04:13,116 --> 00:04:16,196 Speaker 1: and start their own company, right, Like, just that concept, 62 00:04:16,756 --> 00:04:18,636 Speaker 1: I would believe it or not. I mean, I just 63 00:04:18,916 --> 00:04:21,236 Speaker 1: didn't know about that before I came to a m 64 00:04:21,236 --> 00:04:24,236 Speaker 1: I T. I always thought only people that already have 65 00:04:24,916 --> 00:04:28,996 Speaker 1: family money, or like you know that studied business for underground, 66 00:04:29,276 --> 00:04:31,996 Speaker 1: they are the ones who can start businesses. And just 67 00:04:32,116 --> 00:04:35,516 Speaker 1: that concept completely blew my mind. And I think it 68 00:04:35,556 --> 00:04:38,516 Speaker 1: was for me like the missing piece. I realized, Oh, wow, well, 69 00:04:39,356 --> 00:04:41,836 Speaker 1: then that's how I do it. That's how I create 70 00:04:41,916 --> 00:04:43,956 Speaker 1: a company based on science to go and solve a 71 00:04:43,956 --> 00:04:47,196 Speaker 1: big problem. One thing I heard you talking about and 72 00:04:47,276 --> 00:04:52,476 Speaker 1: another interview was when you were starting the company, people 73 00:04:52,596 --> 00:04:57,116 Speaker 1: seem to misunderstand and think that you should be starting 74 00:04:57,116 --> 00:04:59,516 Speaker 1: a nonprofit instead of a company. Is that right and 75 00:04:59,556 --> 00:05:03,196 Speaker 1: if so, can you tell me about that? Absolutely so. 76 00:05:04,076 --> 00:05:09,196 Speaker 1: The idea of selling to government, the idea of of 77 00:05:09,636 --> 00:05:14,956 Speaker 1: operating in the space of public health, and the idea 78 00:05:14,956 --> 00:05:19,516 Speaker 1: of having two women co founders out of MIT basically 79 00:05:19,556 --> 00:05:22,716 Speaker 1: two very technical women co founders. Kind of it was 80 00:05:22,756 --> 00:05:26,716 Speaker 1: a kind of a perfect trifecta for investors to think 81 00:05:26,716 --> 00:05:31,956 Speaker 1: about US as a nonprofit. You mentioned gender, You mentioned 82 00:05:31,996 --> 00:05:33,996 Speaker 1: the fact that that you and your co founder are 83 00:05:33,996 --> 00:05:36,196 Speaker 1: both women, and that that played into it. How did that? 84 00:05:36,796 --> 00:05:41,156 Speaker 1: How did that work? What was going on there? Yeah, 85 00:05:41,436 --> 00:05:44,316 Speaker 1: it seems that we you know, I had to be 86 00:05:44,436 --> 00:05:50,076 Speaker 1: very proactive about kind of explaining I'm doing this not 87 00:05:50,196 --> 00:05:54,796 Speaker 1: just to have public impact, not just to have a 88 00:05:54,876 --> 00:05:58,036 Speaker 1: social impact. I'm doing this because I'm also looking to 89 00:05:58,116 --> 00:06:02,356 Speaker 1: have a financial outcome. And I just found it funny 90 00:06:02,436 --> 00:06:05,156 Speaker 1: that I had to be saying like, hey, I want 91 00:06:05,196 --> 00:06:08,556 Speaker 1: to make money, like you know, I personally also want 92 00:06:08,596 --> 00:06:13,636 Speaker 1: to make any Why wouldn't I? Right, So, okay, so 93 00:06:13,636 --> 00:06:16,876 Speaker 1: so you found the company. So so let's jump to 94 00:06:17,356 --> 00:06:21,676 Speaker 1: say twenty nineteen, like beginning of twenty twenty. Now you 95 00:06:21,716 --> 00:06:23,916 Speaker 1: have this little company that's been up and running for 96 00:06:23,916 --> 00:06:27,156 Speaker 1: a couple of years. It's analyzing wastewater, but you have 97 00:06:27,196 --> 00:06:29,916 Speaker 1: no revenue. You have a staff of just like five people. 98 00:06:30,196 --> 00:06:32,596 Speaker 1: You built this little machine that goes into the sewer 99 00:06:32,676 --> 00:06:36,116 Speaker 1: to collect sewage, and you're working on one pilot project. 100 00:06:36,196 --> 00:06:39,396 Speaker 1: And that project isn't even a fur infectious disease. Right, 101 00:06:39,436 --> 00:06:42,476 Speaker 1: You're looking at opioids, You're you're sort of studying the 102 00:06:42,516 --> 00:06:47,116 Speaker 1: opioid epidemic. And yet this is this moment when history 103 00:06:47,236 --> 00:06:50,716 Speaker 1: is like barreling towards you. Right, this pandemic is about 104 00:06:50,756 --> 00:06:54,156 Speaker 1: to you know, change everything, right, And so I'm curious 105 00:06:54,596 --> 00:06:56,956 Speaker 1: when did you start to hear about, you know, about 106 00:06:56,996 --> 00:07:00,756 Speaker 1: this new coronavirus outbreak. Yeah, I think for us, UM 107 00:07:00,996 --> 00:07:05,836 Speaker 1: it really began to catch our attention in like early 108 00:07:06,036 --> 00:07:10,076 Speaker 1: twenty twenty, like January twenty twenty. Do you remember any 109 00:07:10,156 --> 00:07:14,516 Speaker 1: particular moments, any particular conversations or reading anything or talking 110 00:07:14,516 --> 00:07:19,596 Speaker 1: to anybody. I mean definitely, so some of those like 111 00:07:19,716 --> 00:07:23,916 Speaker 1: first conversations that happened where we had been forming a 112 00:07:23,956 --> 00:07:29,556 Speaker 1: relationship with folks within HHS, like the federal HHS Department 113 00:07:29,556 --> 00:07:31,596 Speaker 1: of Health and Human Services, the big federal Apartment of 114 00:07:31,636 --> 00:07:35,996 Speaker 1: Health and Human Services in DC. So I remember we 115 00:07:35,996 --> 00:07:40,396 Speaker 1: were in DC in February of twenty twenty, basically like 116 00:07:40,436 --> 00:07:43,236 Speaker 1: already pitching that we should begin to test the waste 117 00:07:43,236 --> 00:07:46,556 Speaker 1: water of all of the major airports across the country 118 00:07:46,596 --> 00:07:50,116 Speaker 1: to start getting a sense of the level of import 119 00:07:50,996 --> 00:07:54,676 Speaker 1: And we were told, well, this outbreak is going to 120 00:07:54,756 --> 00:07:58,116 Speaker 1: be contained within two weeks, so it would be a 121 00:07:58,156 --> 00:08:01,436 Speaker 1: waste of your resources to try to develop a solution 122 00:08:01,476 --> 00:08:03,836 Speaker 1: around this now because you're not going to be ready 123 00:08:03,836 --> 00:08:05,516 Speaker 1: in less than two weeks. They were like, by the 124 00:08:05,516 --> 00:08:07,196 Speaker 1: time you figure out how to do this test in 125 00:08:07,196 --> 00:08:09,036 Speaker 1: a few weeks, this all things going to have blown over. 126 00:08:09,396 --> 00:08:12,996 Speaker 1: It's not going exactly so. But despite that sort of 127 00:08:13,036 --> 00:08:17,876 Speaker 1: like very strong feedback, we came back from DC and 128 00:08:17,916 --> 00:08:20,636 Speaker 1: we this was February of twenty twenty, and we make 129 00:08:20,716 --> 00:08:23,196 Speaker 1: the decision that we were going to build a COVID 130 00:08:23,636 --> 00:08:26,876 Speaker 1: solution based on the waste water and tell me about 131 00:08:26,876 --> 00:08:30,756 Speaker 1: building that. We had to basically change everything. We had 132 00:08:30,836 --> 00:08:35,316 Speaker 1: to throw away sort of like all of the progress 133 00:08:35,356 --> 00:08:38,236 Speaker 1: that we had made up until that point to start 134 00:08:38,316 --> 00:08:43,436 Speaker 1: over given the constraints at hand. Number one, this was 135 00:08:43,676 --> 00:08:47,676 Speaker 1: there was a sense of emergency and that time was 136 00:08:47,716 --> 00:08:53,356 Speaker 1: of the essence. So the idea of having to manufacture 137 00:08:53,436 --> 00:08:59,196 Speaker 1: hardware in order to collect data at scale just seemed 138 00:08:59,236 --> 00:09:01,916 Speaker 1: pretty hard or impossible in that moment. Also, do you 139 00:09:01,996 --> 00:09:04,156 Speaker 1: know the supply chain issues all of You're not going 140 00:09:04,156 --> 00:09:05,956 Speaker 1: to be able to make this special device. You're not 141 00:09:05,996 --> 00:09:07,796 Speaker 1: gonna be only like a thousand of them in a 142 00:09:07,836 --> 00:09:11,476 Speaker 1: couple of months or whatever, exactly exactly, So we decided, okay, 143 00:09:11,636 --> 00:09:16,556 Speaker 1: where how else can we get wastewater easily? And we realize, oh, 144 00:09:16,596 --> 00:09:22,396 Speaker 1: actually actually, there are sixteen thousand wastewater treatment plants in 145 00:09:22,436 --> 00:09:26,316 Speaker 1: the US, covering eighty percent of the US population, and 146 00:09:26,956 --> 00:09:30,476 Speaker 1: turns out most of them already have a similar type 147 00:09:30,476 --> 00:09:34,316 Speaker 1: of equipment. So it's not portable, it's not as sophisticated, 148 00:09:35,036 --> 00:09:38,516 Speaker 1: but it does the job at collecting that continues twenty 149 00:09:38,556 --> 00:09:42,676 Speaker 1: four hour sample. The minimum viable product already exists at 150 00:09:42,756 --> 00:09:45,876 Speaker 1: sixteen thousand wastewater plants all across the US, so we 151 00:09:45,876 --> 00:09:50,876 Speaker 1: were like, okay, check. Second was the lab component. We 152 00:09:50,956 --> 00:09:55,556 Speaker 1: had been building a chemistry platform, and obviously and COVID 153 00:09:55,836 --> 00:10:00,876 Speaker 1: is caused by an RNA virus, so the detection or 154 00:10:00,916 --> 00:10:02,996 Speaker 1: detest in the lab would need to be more of 155 00:10:03,036 --> 00:10:06,156 Speaker 1: a molecular biology type of approach. We didn't have any 156 00:10:06,196 --> 00:10:12,756 Speaker 1: of those capabilities, but we had raised seat round, like 157 00:10:12,796 --> 00:10:15,756 Speaker 1: a you know, a four point two million dollars seat round, 158 00:10:16,276 --> 00:10:19,636 Speaker 1: and so we had some money to just start getting 159 00:10:19,676 --> 00:10:23,636 Speaker 1: some basic new equipment in the lab. And also we 160 00:10:23,676 --> 00:10:28,036 Speaker 1: have a good relationship with my PhD advisor at MT 161 00:10:28,876 --> 00:10:32,876 Speaker 1: so together with him, we actually used a lot of 162 00:10:33,116 --> 00:10:36,436 Speaker 1: his lab early on in the pandemic and collaborated with 163 00:10:36,556 --> 00:10:39,836 Speaker 1: his students. So you know, that's how we got started, 164 00:10:39,916 --> 00:10:45,916 Speaker 1: and it took us like four weeks to just go 165 00:10:46,116 --> 00:10:50,676 Speaker 1: from the decision point until having actually not just a 166 00:10:50,796 --> 00:10:56,756 Speaker 1: proof that the test works with waste water, but also 167 00:10:57,196 --> 00:11:02,436 Speaker 1: we wrote a scientific preprint, like a you know, a paper. 168 00:11:02,756 --> 00:11:04,796 Speaker 1: So we were the first ones in the country to 169 00:11:05,236 --> 00:11:10,636 Speaker 1: demonstrate that you can detect and quantify the virus causing 170 00:11:10,636 --> 00:11:14,716 Speaker 1: COVID nineteen in wastewater samples and that it works well. 171 00:11:15,956 --> 00:11:18,116 Speaker 1: And I think, you know, it was just from there 172 00:11:18,156 --> 00:11:21,676 Speaker 1: that everything exploded. I mean, is there fear I assume 173 00:11:21,756 --> 00:11:25,756 Speaker 1: that that there's no risk of transmitting the disease. I mean, 174 00:11:25,756 --> 00:11:30,276 Speaker 1: it's obviously not transmitted through wastewater, but we're people afraid 175 00:11:30,316 --> 00:11:32,436 Speaker 1: that it might be. We didn't know anything about COVID 176 00:11:32,476 --> 00:11:33,796 Speaker 1: at the time. I mean, this is when I was 177 00:11:33,796 --> 00:11:37,076 Speaker 1: still wiping off all my groceries with bleach, right, because 178 00:11:37,236 --> 00:11:40,516 Speaker 1: literally we didn't know. Oh yes, there was a lot 179 00:11:40,556 --> 00:11:43,516 Speaker 1: of concern. And actually that was you know, thank you 180 00:11:43,596 --> 00:11:46,636 Speaker 1: for I feel like my brain probably like tried to 181 00:11:46,676 --> 00:11:50,196 Speaker 1: forget that, but that was a very big deal. Like 182 00:11:50,236 --> 00:11:54,196 Speaker 1: when we just started from for many reasons, nobody knew 183 00:11:54,956 --> 00:11:58,796 Speaker 1: if the virus present in wastewater would be infectious. My 184 00:11:59,076 --> 00:12:02,796 Speaker 1: very small team that we had. They were really afraid 185 00:12:03,116 --> 00:12:06,636 Speaker 1: to have to handle the wastewater sample without knowing if 186 00:12:06,676 --> 00:12:10,036 Speaker 1: they can get infected. So we came up with a 187 00:12:10,076 --> 00:12:15,676 Speaker 1: solution for our lab where every every bottle of wastewater, 188 00:12:15,676 --> 00:12:19,756 Speaker 1: example that arrives to buy a boat is first pasteurized 189 00:12:20,756 --> 00:12:25,996 Speaker 1: in order to basically make it non infectious or like sterile. Yes. 190 00:12:26,116 --> 00:12:28,876 Speaker 1: And the third thing was that I had just I 191 00:12:28,916 --> 00:12:32,356 Speaker 1: was just pregnant at the time. I became pregnant in 192 00:12:32,396 --> 00:12:36,516 Speaker 1: March of twenty twenty, my first pregnancy, my first you 193 00:12:36,556 --> 00:12:40,836 Speaker 1: know baby, and I my husband and I also would 194 00:12:40,876 --> 00:12:43,916 Speaker 1: go into the lab to model to the rest of 195 00:12:43,956 --> 00:12:47,676 Speaker 1: our team that you know this is safe, Like you know, 196 00:12:47,796 --> 00:12:53,156 Speaker 1: I'm pregnant, but I trust that the pasteurization is good 197 00:12:53,276 --> 00:12:56,476 Speaker 1: enough to make this work safe and let me, you know, 198 00:12:56,596 --> 00:12:59,556 Speaker 1: let me demonstrate that with my actions. At the time, 199 00:12:59,676 --> 00:13:02,796 Speaker 1: it was a very big deal. So you have this idea, 200 00:13:03,236 --> 00:13:06,356 Speaker 1: you have the proof that it works, You've convinced the 201 00:13:06,396 --> 00:13:08,796 Speaker 1: staff at your company, at Bio Boat, that it's okay 202 00:13:08,836 --> 00:13:11,996 Speaker 1: to do this. How do you get customers what happens next? 203 00:13:12,316 --> 00:13:15,036 Speaker 1: So we designed a probono campaign where by About would 204 00:13:15,036 --> 00:13:20,716 Speaker 1: absorb the cost of all of the testing for two months, 205 00:13:21,036 --> 00:13:24,636 Speaker 1: and what happened it was beyond our wildest dreams, Like 206 00:13:25,316 --> 00:13:30,516 Speaker 1: it exploded. It got picked up by the media. People 207 00:13:30,836 --> 00:13:38,236 Speaker 1: loft this idea that you can fight COVID with poop. Poop. Yeah, 208 00:13:38,236 --> 00:13:40,716 Speaker 1: you can fight COVID with poop. You know. That was 209 00:13:40,796 --> 00:13:43,196 Speaker 1: that was basically the message being put out there. It 210 00:13:43,276 --> 00:13:48,716 Speaker 1: went viral and we got in just the first ten 211 00:13:48,836 --> 00:13:52,596 Speaker 1: days or so of announcing the campaign, we got four 212 00:13:52,756 --> 00:13:58,396 Speaker 1: hundred plants nationwide wanting to enroll in the program. We 213 00:13:58,436 --> 00:14:03,116 Speaker 1: had aspired to enroll up to one hundred, So it 214 00:14:03,196 --> 00:14:07,036 Speaker 1: was it was crazy. The response was just crazy. But 215 00:14:07,196 --> 00:14:09,836 Speaker 1: you know, that transition was tough because I guess also 216 00:14:09,876 --> 00:14:13,236 Speaker 1: something that I haven't talked about is that in that 217 00:14:13,356 --> 00:14:17,116 Speaker 1: early twenty twenty stage, when we were the first ones, 218 00:14:17,716 --> 00:14:23,996 Speaker 1: the more established academics were skeptical and basically like recommending 219 00:14:24,956 --> 00:14:28,676 Speaker 1: caution with these and slowing things down for us. And 220 00:14:29,116 --> 00:14:32,756 Speaker 1: so it was a very Were they skeptical of wastewater 221 00:14:32,876 --> 00:14:37,356 Speaker 1: sampling in general or were they skeptical of your company? 222 00:14:37,876 --> 00:14:44,836 Speaker 1: Or was it both both both? So, up until twenty twenty, 223 00:14:45,516 --> 00:14:51,796 Speaker 1: the wastewater epidemiology was a very niche and obscure area 224 00:14:51,836 --> 00:14:55,796 Speaker 1: of science, So the entire field was a little bit like, 225 00:14:56,636 --> 00:15:01,156 Speaker 1: you know, not very well known obviously. And if that 226 00:15:01,356 --> 00:15:04,956 Speaker 1: was within science, just imagine within government, you know, like 227 00:15:04,956 --> 00:15:07,956 Speaker 1: it was just nobody was saying, oh, I need wastewater data, 228 00:15:08,036 --> 00:15:10,796 Speaker 1: like who can provide it for me? Why do you 229 00:15:10,836 --> 00:15:12,916 Speaker 1: think that is? I mean, do you think it's partly 230 00:15:12,956 --> 00:15:16,396 Speaker 1: like because it's poop, because it's sewage? Like why do 231 00:15:16,436 --> 00:15:19,836 Speaker 1: you think people were ignoring this source of data? Really? 232 00:15:20,036 --> 00:15:22,956 Speaker 1: I mean, is it because it's it's poop and people 233 00:15:22,956 --> 00:15:24,756 Speaker 1: are kind of embarrassed by it don't like to talk 234 00:15:24,796 --> 00:15:28,156 Speaker 1: about it? Like has that been a barrier too? I 235 00:15:28,196 --> 00:15:31,036 Speaker 1: mean I think that No. I think that actually people 236 00:15:31,196 --> 00:15:35,436 Speaker 1: love all of the poop jokes and poop humor. I 237 00:15:35,476 --> 00:15:38,276 Speaker 1: feel like that actually tends to be like a plus. 238 00:15:38,276 --> 00:15:40,836 Speaker 1: How do you feel about them at this point? Like 239 00:15:40,916 --> 00:15:43,156 Speaker 1: are you tired of them? Do they work in your favor? 240 00:15:43,596 --> 00:15:45,556 Speaker 1: Like our poop jokes like enough, I never want to 241 00:15:45,556 --> 00:15:47,796 Speaker 1: hear a poop joke again? No, no, no, I love it. 242 00:15:47,836 --> 00:15:50,636 Speaker 1: I love it. And our company culture, you know, celebrates 243 00:15:50,676 --> 00:15:55,276 Speaker 1: the poop humor quite a lot with okay, our you know, 244 00:15:55,396 --> 00:15:59,236 Speaker 1: branding and suave and you know, we we love it. 245 00:15:59,396 --> 00:16:01,716 Speaker 1: We love the users of the poop emoji or they're 246 00:16:01,756 --> 00:16:05,276 Speaker 1: like particularly very big. Oh yeah, the poop emoji is 247 00:16:05,276 --> 00:16:09,556 Speaker 1: the most use emoji in the bay about slack, it 248 00:16:09,676 --> 00:16:12,116 Speaker 1: makes sense. I mean, it's why they're used even at 249 00:16:12,156 --> 00:16:15,836 Speaker 1: places that have nothing to do with it. Right, So yes, no, 250 00:16:15,956 --> 00:16:17,876 Speaker 1: I think that, you know, I think that the barrier 251 00:16:18,796 --> 00:16:22,516 Speaker 1: to research and to you know, first to research and 252 00:16:22,556 --> 00:16:26,556 Speaker 1: then to the adoption of the data is just that 253 00:16:26,636 --> 00:16:30,156 Speaker 1: the bar to get the data in is very high 254 00:16:30,236 --> 00:16:33,516 Speaker 1: in the sense that there's a lot of operational and 255 00:16:33,676 --> 00:16:38,836 Speaker 1: logistical work in order to get data. So when you 256 00:16:38,876 --> 00:16:43,116 Speaker 1: are working within a university or within a government agency, 257 00:16:43,796 --> 00:16:47,396 Speaker 1: who really has the time to be you know, building 258 00:16:47,676 --> 00:16:51,556 Speaker 1: kits to transport wastewater, learning how to the regulations to 259 00:16:51,636 --> 00:16:56,516 Speaker 1: transport it going out there, or finding the relationships to 260 00:16:56,556 --> 00:16:59,316 Speaker 1: send you the waste water in doing all of that 261 00:16:59,476 --> 00:17:03,316 Speaker 1: groundwork in the lab, right groundwork. Right, It's very logistical. 262 00:17:03,556 --> 00:17:06,716 Speaker 1: It's not like high minded, it's not fancy math. It's 263 00:17:06,796 --> 00:17:11,236 Speaker 1: just getting appealed to the lab. Yeah, exactly, exactly. So 264 00:17:11,316 --> 00:17:13,356 Speaker 1: when I was a PITCHD student that in my t 265 00:17:14,796 --> 00:17:19,756 Speaker 1: I was basically like the only one like willing to 266 00:17:19,796 --> 00:17:23,876 Speaker 1: do the groundwork because I started working on always what 267 00:17:24,076 --> 00:17:27,476 Speaker 1: epidemiology during my PhD. That's what my pitchd PSIS was about. 268 00:17:28,476 --> 00:17:32,876 Speaker 1: But you know, I really struggled with just like recruiting 269 00:17:32,916 --> 00:17:37,276 Speaker 1: a team around me, because folks were wanting to help 270 00:17:37,316 --> 00:17:41,516 Speaker 1: with the data analysis, the data visualization, but not the 271 00:17:41,596 --> 00:17:44,996 Speaker 1: eighty percent groundwork that goes behind it. So nobody wants 272 00:17:45,556 --> 00:17:49,556 Speaker 1: get the proof exactly. So that's why I thought, always thought, 273 00:17:49,596 --> 00:17:57,316 Speaker 1: you know, if this science is going to really go mainstream, 274 00:17:57,436 --> 00:18:02,036 Speaker 1: it needs an organization dedicated like one to it so 275 00:18:02,036 --> 00:18:05,356 Speaker 1: that we can do that around work and then the scientists. Yes, 276 00:18:05,516 --> 00:18:08,316 Speaker 1: of course, the power is in the data, in the 277 00:18:08,356 --> 00:18:10,396 Speaker 1: insights that you can get out of it, but you 278 00:18:10,436 --> 00:18:12,876 Speaker 1: know there's an eighty percent around work behind it. Well, 279 00:18:12,916 --> 00:18:14,796 Speaker 1: I mean maybe that's part of the case for a 280 00:18:14,796 --> 00:18:18,756 Speaker 1: for profit company, right Like profit is a very good 281 00:18:18,796 --> 00:18:23,996 Speaker 1: incentive to do groundwork, absolutely absolutely Well. How big is 282 00:18:24,036 --> 00:18:28,876 Speaker 1: the company now? Oh yeah, buy about now has one 283 00:18:28,956 --> 00:18:31,756 Speaker 1: hundred employees. How much revenue do you have? More or less? 284 00:18:32,396 --> 00:18:37,956 Speaker 1: This year? We are on track to make over twenty 285 00:18:37,956 --> 00:18:41,956 Speaker 1: million dollars in revenue, okay, which is great also to 286 00:18:42,036 --> 00:18:44,516 Speaker 1: be here given that two years ago we were basically 287 00:18:44,596 --> 00:18:47,796 Speaker 1: pre revenue. So yes, two years ago was zero. From 288 00:18:47,876 --> 00:18:53,756 Speaker 1: zero to twenty million, there's a lot. Are you profitable, No, 289 00:18:53,996 --> 00:18:57,996 Speaker 1: not yet. I mean we are investing heavily in our 290 00:18:58,156 --> 00:19:00,476 Speaker 1: R and D so that we can look at other 291 00:19:00,516 --> 00:19:06,676 Speaker 1: types of data. So story so far. Three years ago, 292 00:19:07,156 --> 00:19:10,156 Speaker 1: Mariana barely had a company, and waste water surveillance was 293 00:19:10,196 --> 00:19:14,076 Speaker 1: this little niche thing in the US. And then by 294 00:19:14,116 --> 00:19:16,956 Speaker 1: the beginning of this year, when the omicron wave hit 295 00:19:16,996 --> 00:19:21,276 Speaker 1: the country, BAA was this big, real company. Wastewater surveillance 296 00:19:21,356 --> 00:19:24,156 Speaker 1: was one of the most important tools public health officials 297 00:19:24,236 --> 00:19:27,116 Speaker 1: used to figure out where cases were spiking, so that 298 00:19:27,156 --> 00:19:29,956 Speaker 1: they could direct resources into the right neighborhoods and you know, 299 00:19:30,276 --> 00:19:33,956 Speaker 1: plan for surges and hospital admissions. And the story is 300 00:19:33,996 --> 00:19:37,396 Speaker 1: not over in a minute. What we still don't know 301 00:19:37,596 --> 00:19:48,356 Speaker 1: about poop and disease. It's a lot. Now back to 302 00:19:48,396 --> 00:19:52,396 Speaker 1: the show. And one thing I'm curious about, Well, what 303 00:19:52,596 --> 00:19:55,036 Speaker 1: is your company's moat? Right? Like, how is what you 304 00:19:55,156 --> 00:19:57,956 Speaker 1: do not just some commodity thing that anybody with the 305 00:19:58,036 --> 00:20:04,236 Speaker 1: lab can do. Yeah, I think that that's a great question. Everybody, anybody, 306 00:20:04,276 --> 00:20:09,036 Speaker 1: anybody out there could go and let's say open amunthole 307 00:20:10,516 --> 00:20:14,796 Speaker 1: and get a bucket in pull up wastewater, take it 308 00:20:14,796 --> 00:20:18,196 Speaker 1: to a lab and they would get a number, a 309 00:20:18,356 --> 00:20:20,996 Speaker 1: number meaning like an amount of COVID that's in there 310 00:20:21,076 --> 00:20:23,716 Speaker 1: or something. You may get an amount of COVID, you make, 311 00:20:23,916 --> 00:20:27,236 Speaker 1: an amount of opioids, an amount of influenza, right like 312 00:20:27,596 --> 00:20:30,836 Speaker 1: any any number. You may get a number, But what 313 00:20:30,876 --> 00:20:36,516 Speaker 1: does that number mean? Like that number in isolation really 314 00:20:36,556 --> 00:20:42,196 Speaker 1: doesn't mean anything. So our secret sauce is how do 315 00:20:42,276 --> 00:20:48,716 Speaker 1: we create how do we make wastewater a data platform? 316 00:20:48,756 --> 00:20:53,996 Speaker 1: And that really means it's a systems level type of 317 00:20:54,036 --> 00:20:58,396 Speaker 1: design and product. We actually need to use the same 318 00:20:58,436 --> 00:21:03,996 Speaker 1: method in every sample. If we want to calculate, if 319 00:21:03,996 --> 00:21:07,436 Speaker 1: we want to compare across time, if we want to 320 00:21:07,476 --> 00:21:13,716 Speaker 1: compare across locations, we want to build a state statewide trend, 321 00:21:13,756 --> 00:21:16,156 Speaker 1: if we want to compare regions, if we want to 322 00:21:16,236 --> 00:21:21,036 Speaker 1: calculate a national average, it's imperative to actually use the 323 00:21:21,156 --> 00:21:26,716 Speaker 1: same methods at every stage for every sample. Otherwise you 324 00:21:26,756 --> 00:21:30,436 Speaker 1: don't have that comparability wastewater. Again, the power of the 325 00:21:30,476 --> 00:21:34,356 Speaker 1: wastewater isn't about what you about the number that you 326 00:21:34,396 --> 00:21:38,636 Speaker 1: get from a single wastewater sample. The power of wastewater 327 00:21:38,756 --> 00:21:43,076 Speaker 1: is about having that bird's eye view, that systems level 328 00:21:43,156 --> 00:21:46,196 Speaker 1: view of what's happening in an entire country, in an 329 00:21:46,316 --> 00:21:50,956 Speaker 1: entire state. You can drill in more geographically narrow, but 330 00:21:51,036 --> 00:21:54,316 Speaker 1: you can also zoom out. That's the platform, that's the 331 00:21:54,356 --> 00:21:57,596 Speaker 1: power that we're building. So in order to accomplish that, 332 00:21:58,316 --> 00:22:01,876 Speaker 1: you know, that's where we come in, all of the relationships, 333 00:22:01,956 --> 00:22:07,596 Speaker 1: all of the scale that eight you know, groundwork that 334 00:22:07,636 --> 00:22:12,316 Speaker 1: I talked about. To accomplish that level of visibility, that's 335 00:22:12,316 --> 00:22:14,836 Speaker 1: a tough problem. So it's like a network effect. There's 336 00:22:14,836 --> 00:22:17,556 Speaker 1: a network effect thing people would want to use your 337 00:22:17,596 --> 00:22:20,756 Speaker 1: company because you have the most data and therefore you 338 00:22:20,916 --> 00:22:24,796 Speaker 1: have the best understanding of what one particular sample means 339 00:22:24,836 --> 00:22:26,516 Speaker 1: because you can put it in the context of all 340 00:22:26,516 --> 00:22:30,876 Speaker 1: the other data that you have gotten, have processed exactly exactly. 341 00:22:30,996 --> 00:22:35,516 Speaker 1: So we have the largest network of sites in the world. 342 00:22:36,796 --> 00:22:40,716 Speaker 1: It's mostly US, some Canada. How big is it now? 343 00:22:41,596 --> 00:22:45,516 Speaker 1: We have over five hundred sites. What's something you haven't 344 00:22:45,516 --> 00:22:48,236 Speaker 1: figured out yet? What's a problem your story of still 345 00:22:48,276 --> 00:22:50,156 Speaker 1: trying to figure out how to solve Maybe you've tried 346 00:22:50,196 --> 00:22:52,556 Speaker 1: it hasn't quite worked yet. What's something you're still working 347 00:22:52,596 --> 00:22:56,676 Speaker 1: on in the lab. There is a lot of interest 348 00:22:56,756 --> 00:23:01,196 Speaker 1: in looking at different targets, so not just COVID, but 349 00:23:01,276 --> 00:23:06,876 Speaker 1: also influenza, neural virus. You can you know, at least 350 00:23:06,956 --> 00:23:10,556 Speaker 1: keeps going right like monkey box where right now in 351 00:23:10,636 --> 00:23:13,396 Speaker 1: advanced R and D for monkey pops, we're gonna have 352 00:23:13,396 --> 00:23:16,036 Speaker 1: a start testing with water for monkey pops too. But 353 00:23:16,956 --> 00:23:20,356 Speaker 1: it remains a challenge to know kind of how to 354 00:23:21,236 --> 00:23:23,516 Speaker 1: which types of data we can collect and how to 355 00:23:23,556 --> 00:23:29,996 Speaker 1: interpret them because we don't really know which pathogens are 356 00:23:30,676 --> 00:23:36,276 Speaker 1: shed in poop. Huh, that's surprising. I would think people 357 00:23:36,316 --> 00:23:38,076 Speaker 1: would just sort of have figured that out by now. 358 00:23:38,196 --> 00:23:39,556 Speaker 1: Is that Does that go back to the fact that 359 00:23:39,636 --> 00:23:44,716 Speaker 1: it's kind of an understudied area exactly exactly. There's a 360 00:23:44,756 --> 00:23:48,316 Speaker 1: lot of information about how you can diagnose a disease 361 00:23:48,556 --> 00:23:52,996 Speaker 1: in a person via like a serom sample, via maybe 362 00:23:53,036 --> 00:23:57,116 Speaker 1: a blood test, yeah, a blood test, or maybe a 363 00:23:57,716 --> 00:24:00,556 Speaker 1: you know, a swab or like a you know, a 364 00:24:00,596 --> 00:24:05,036 Speaker 1: nasal swab or a saliva suab sometimes in in p 365 00:24:05,796 --> 00:24:09,596 Speaker 1: But but pooh, I mean, why would they have to 366 00:24:09,676 --> 00:24:12,796 Speaker 1: I mean, the clinic the worst way to get us 367 00:24:12,836 --> 00:24:15,436 Speaker 1: to a sample for the doctor, right exactly, So so 368 00:24:15,476 --> 00:24:18,316 Speaker 1: for the doctor like you know, again, poop is an 369 00:24:18,396 --> 00:24:23,196 Speaker 1: understudied like sort of like diagnostic matrix. So you know, 370 00:24:23,276 --> 00:24:26,476 Speaker 1: one wild dream of mine is for us to develop 371 00:24:27,076 --> 00:24:31,156 Speaker 1: the clinical collaborations to to be able to know in 372 00:24:31,276 --> 00:24:36,236 Speaker 1: people like how different diseases are um shedding in poop, 373 00:24:36,356 --> 00:24:39,676 Speaker 1: because that that will help us to understand which targets 374 00:24:39,676 --> 00:24:44,036 Speaker 1: will be easier to track via the waste water and 375 00:24:44,156 --> 00:24:47,916 Speaker 1: how to interpret it. You mentioned sort of starting out 376 00:24:47,956 --> 00:24:52,196 Speaker 1: in the field with this the dream of being able 377 00:24:52,276 --> 00:24:56,956 Speaker 1: to to look at a city like Mexico City and 378 00:24:57,036 --> 00:24:58,916 Speaker 1: get you know, sort of a map of what's going 379 00:24:58,956 --> 00:25:02,076 Speaker 1: on with the health of people in the city. How 380 00:25:02,076 --> 00:25:03,796 Speaker 1: do you get to there from where you are now? 381 00:25:04,276 --> 00:25:08,356 Speaker 1: Because we have done some pilots in Mexico Ecuador Ry 382 00:25:09,476 --> 00:25:12,756 Speaker 1: in partnership already with a World Bank, so you know, 383 00:25:12,916 --> 00:25:14,796 Speaker 1: it's kind of how do we build on top of 384 00:25:14,836 --> 00:25:18,636 Speaker 1: those early pilots that were very successful. So you sample, 385 00:25:18,916 --> 00:25:22,916 Speaker 1: you know, big sewage systems in the US that allows 386 00:25:22,916 --> 00:25:26,236 Speaker 1: you to capture something like eighty percent of the population, 387 00:25:26,716 --> 00:25:29,516 Speaker 1: but it seems like in large parts of the developing 388 00:25:29,556 --> 00:25:32,636 Speaker 1: world you have places you know, that are less developed, 389 00:25:32,676 --> 00:25:35,116 Speaker 1: where where there are not sewage systems. What do you 390 00:25:35,156 --> 00:25:39,356 Speaker 1: do there. There's many areas that don't have any sort 391 00:25:39,356 --> 00:25:44,356 Speaker 1: of sanitation, right, so I think that for those areas 392 00:25:45,076 --> 00:25:48,956 Speaker 1: there's just immediate action, which is we can use basically 393 00:25:49,036 --> 00:25:54,156 Speaker 1: the same type of sampling approaches to open sewers, like 394 00:25:54,196 --> 00:25:58,036 Speaker 1: basically which are rivers where the waste makes it into 395 00:25:58,076 --> 00:26:01,276 Speaker 1: the river, and that that is possible, that that works, 396 00:26:01,316 --> 00:26:04,156 Speaker 1: that's how some people already do waste. What are epithimolity 397 00:26:04,156 --> 00:26:07,756 Speaker 1: You work in areas without the infrastructure. But I think 398 00:26:07,756 --> 00:26:13,516 Speaker 1: you're right at over time this technology could be a 399 00:26:13,596 --> 00:26:17,156 Speaker 1: new reason to invest in waste, what in building the 400 00:26:17,196 --> 00:26:21,436 Speaker 1: waste water infrastructure where it doesn't exist, because now we're 401 00:26:21,476 --> 00:26:26,076 Speaker 1: giving it a second second use, a second mission. This 402 00:26:26,156 --> 00:26:29,796 Speaker 1: is no longer just about the collection and safe disposal 403 00:26:29,876 --> 00:26:33,836 Speaker 1: of waste from people. This is about intelligence that you 404 00:26:33,876 --> 00:26:38,316 Speaker 1: cannot really get any in any other way. So hopefully 405 00:26:38,316 --> 00:26:42,556 Speaker 1: it can be a virtual cycle where it can foster 406 00:26:43,396 --> 00:26:47,836 Speaker 1: the investment in the building of this infrastructure, which would 407 00:26:47,836 --> 00:26:52,516 Speaker 1: only of course also improve public health because then would 408 00:26:52,516 --> 00:26:59,396 Speaker 1: reduce the spread of disease. In a minute, the lightning 409 00:26:59,436 --> 00:27:04,076 Speaker 1: round with questions about immigration, entrepreneurship, and what not to 410 00:27:04,196 --> 00:27:15,196 Speaker 1: flush down the toilet now back to the show. Let's 411 00:27:15,236 --> 00:27:18,036 Speaker 1: just do let's do like a lightning round. I'm just 412 00:27:18,076 --> 00:27:19,996 Speaker 1: gonna ask you a bunch of fast questions and you 413 00:27:20,036 --> 00:27:24,116 Speaker 1: can answer them fast. Why do you think immigrants are 414 00:27:24,156 --> 00:27:30,156 Speaker 1: more likely than native born citizens to become entrepreneurs? That's 415 00:27:30,156 --> 00:27:32,316 Speaker 1: a good question, so I I mean, I can only 416 00:27:32,356 --> 00:27:35,956 Speaker 1: speak about my kind of experience, but at least in 417 00:27:35,956 --> 00:27:39,636 Speaker 1: my case, I felt that a little bit like I 418 00:27:39,676 --> 00:27:44,316 Speaker 1: had nothing to lose, So why not try? Like, why 419 00:27:44,316 --> 00:27:48,436 Speaker 1: wouldn't I try? What's one thing people should never ever 420 00:27:48,596 --> 00:27:59,836 Speaker 1: flush down the toilet? Well, that's a great question. Never 421 00:27:59,996 --> 00:28:06,596 Speaker 1: flushed down the so called flushable wipes okay, not actually 422 00:28:06,836 --> 00:28:13,556 Speaker 1: flushable at do not actually flushable. They don't dissolve in water, 423 00:28:14,356 --> 00:28:18,876 Speaker 1: So what happens is that they create this massive the 424 00:28:19,116 --> 00:28:23,276 Speaker 1: glock systems everywhere. The Q tips are also pretty bad 425 00:28:23,396 --> 00:28:26,876 Speaker 1: because they have size tends to be the same as 426 00:28:26,996 --> 00:28:30,836 Speaker 1: like some of the sort of like filters that capture 427 00:28:31,316 --> 00:28:33,716 Speaker 1: just like big, big junk out of the waste water, 428 00:28:33,756 --> 00:28:37,036 Speaker 1: so they also block those systems. The Q tips are 429 00:28:37,076 --> 00:28:41,436 Speaker 1: not great to fulush either. Feel like I touch the 430 00:28:41,556 --> 00:28:46,916 Speaker 1: nerve here? Yes? Oh yeah? If everything goes well, what 431 00:28:47,076 --> 00:28:49,236 Speaker 1: problem will you be trying to solve in five years, 432 00:28:50,756 --> 00:28:55,076 Speaker 1: due to climate change, we can expect to see more 433 00:28:55,996 --> 00:29:00,996 Speaker 1: infectious outbreaks than before in a more frequent manner. The 434 00:29:01,036 --> 00:29:07,596 Speaker 1: patterns of you know, just wildlife of insects, factor insects 435 00:29:07,596 --> 00:29:12,756 Speaker 1: are just changing, so so we can expect to see 436 00:29:12,796 --> 00:29:17,876 Speaker 1: more zonoric events of infectious diseases jumping to humans. So, 437 00:29:18,756 --> 00:29:20,836 Speaker 1: in my opinion, I think that the next five to 438 00:29:20,956 --> 00:29:25,356 Speaker 1: ten years should be more about that international collaboration around 439 00:29:25,356 --> 00:29:29,396 Speaker 1: health understanding. You know, what we do here won't be enough. 440 00:29:29,556 --> 00:29:32,476 Speaker 1: We need to be connected. So I would love if 441 00:29:32,996 --> 00:29:35,916 Speaker 1: we have been so successful at collecting, you know, at 442 00:29:35,916 --> 00:29:40,116 Speaker 1: building this data asset and having the trust of different 443 00:29:40,156 --> 00:29:43,236 Speaker 1: governments that we can even begin to be part of 444 00:29:43,276 --> 00:29:48,516 Speaker 1: that collaboration. What's the most interesting thing you ever saw 445 00:29:48,756 --> 00:29:52,196 Speaker 1: pulled out of a sewer? Oh? Wow, Yeah, that's another 446 00:29:52,276 --> 00:29:59,076 Speaker 1: good one. Well, I mean I didn't pull this myself, 447 00:29:59,436 --> 00:30:03,276 Speaker 1: but we were touring one of the plants that we 448 00:30:03,396 --> 00:30:07,396 Speaker 1: work with in in Portland, Maine just a few weeks ago, 449 00:30:08,076 --> 00:30:12,356 Speaker 1: and they were telling us that they recently pulled the 450 00:30:12,436 --> 00:30:19,116 Speaker 1: door of a car. I don't know what happened, but 451 00:30:19,196 --> 00:30:22,156 Speaker 1: there was a door of a car that they had 452 00:30:22,196 --> 00:30:30,796 Speaker 1: to pull out of the stream. Marianna Matus is co 453 00:30:30,916 --> 00:30:35,596 Speaker 1: founder and CEO of biobot Analytics. Today's show was produced 454 00:30:35,596 --> 00:30:39,276 Speaker 1: by Edith Ruslow, engineered by Amanda ka Wong, and edited 455 00:30:39,276 --> 00:30:42,276 Speaker 1: by Robert Smith. I'm Jacob Goldstein, and we'll be back 456 00:30:42,316 --> 00:30:53,116 Speaker 1: next week with another episode of What's Your Problem